Differential expression of KCNQ1 K+ channel in tubular cells of frog kidney |
| |
| Authors: | S. ?irovi? J. Markovi?-Lipkovski J. Todorovi? J. Ne?ovi?-Ostoji? M. Jovi? S. Ili? S. Tati? D. ?emeriki? |
| |
| Affiliation: | 1.Institute of Pathology, Medical Faculty, Belgrade, Serbia;;2.Institute of Pathological Physiology, Medical Faculty, Belgrade, Serbia;;3.Center for Pathology and Forensic Medicine, Military Medical Academy, Belgrade, Serbia |
| |
| Abstract: | The aim of this study was to evaluate KCNQ1 K+ channel expression in the frog kidney of Rana esculenta. KCNQ1 K+ channel, also known as KvLQT1, is the pore forming α-subunit of the IKs K+ channel, a delayed rectifier voltage-gated K+ channel, which has an important role in water and salt transport in the kidney and gastrointestinal tract. The expression of KCNQ1 K+ channel along tubular epithelium differs from species to species. In the present study the expression of KCNQ1 K+ channel in the frog kidney has been demonstrated by immunohistochemistry. The presence of KCNQ1 K+ channel was demonstrated in the epithelial cells of distal convoluted tubule and collecting duct. However, the pattern of expression of KCNQ1 K+ channel differs between distal convoluted tubules and collecting duct. All epithelial cells of distal convoluted tubules revealed basolateral expression of KCNQ1 K+ channel. On the contrary, only the single cells of collecting duct, probably intercalated cells, showed diffuse cell surface staining with antibodies against KCNQ1 K+ channel. These findings suggest that KCNQ1 K+ channel has cell-specific roles in renal potassium ion transport.Key words: KCNQ1 K+ channel, rana esculenta, frog kidney, immunohistochemistry. |
| |
| Keywords: | |
|
|
