| 简单快速的DNA银染和胶保存方法 |
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| 引用本文： | 许绍斌,陶玉芬,杨昭庆,褚嘉档. 简单快速的DNA银染和胶保存方法[J]. 遗传, 2002, 24(3): 335-336 |
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| 作者姓名： | 许绍斌 陶玉芬 杨昭庆 褚嘉档 |
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| 作者单位： | 中国医学科学院 中国协和医科大学医学生物学研究所遗传室,昆明650118Department of Genetics,Institute of Medical Biology,Chinese Academy of Medical Sciences& Peking Union Medical College,Kunming 650118,China |
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| 基金项目： | 国家自然科学基金重大项目 ( 3999342 0 ) |
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| 摘 要： | 本文介绍了一套简单快速的DNA银染以及胶保存的方法,整个过程仅需10~15分钟,而且背景浅,条带清楚,灵敏度高,稳定性好。胶保存采用双层玻璃纸夹心法,可长久地保存胶显色时的原貌。以常规PAG胶检测和HLA的SSCP分型为例,利用该套方法进行了银染以及胶的保存,均得到了满意的结果。该方法具有推广价值。Abstract:This paper introduced the simple and rapid methods of silver staining and gel preservation.It was taken only about 10 and 15 minutes to stain a gel.The background of gel was light,the bands were clear,the sensibility was high and the stabilization was well by the method of silver staining.The gel preservation adopted a method named two-layer transparent plastic paper "Sandwich" which could keep the gel with primitive colors for a long time.The methods were used on PAG checking and SSCP typing of HLA and the results were satisfactory.The set of methods are expected to be widely used in laboratories.
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| 关 键 词： | 银染 胶保存 PAG SSCP Key words silver staining,gel preservation,PAG,SSCP, |
| 文章编号： | 0253-9772(2002)03-0335-02 |
| 修稿时间： | 2001-10-26 |
A Simple and Rapid Methods Used for Silver Staining and Gel Preservation |
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| XU Shao bin,TAO Yu fen,YANG Zhao qing,CHU Jia you. A Simple and Rapid Methods Used for Silver Staining and Gel Preservation[J]. Hereditas, 2002, 24(3): 335-336 |
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| Authors: | XU Shao bin TAO Yu fen YANG Zhao qing CHU Jia you |
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| Affiliation: | Department of Genetics, Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, China. xushaobin@yahoo.com |
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| Abstract: | This paper introduced the simple and rapid methods of silver staining and gel preservation.It was taken only about 10 and 15 minutes to stain a gel.The background of gel was light,the bands were clear,the sensibility was high and the stabilization was well by the method of silver staining.The gel preservation adopted a method named two-layer transparent plastic paper "Sandwich" which could keep the gel with primitive colors for a long time.The methods were used on PAG checking and SSCP typing of HLA and the results were satisfactory.The set of methods are expected to be widely used in laboratories. |
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| Keywords: | silver staining gel preservation PAG SSCP |
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