Verification of the intermolecular parallel beta-sheet in E22K-Abeta42 aggregates by solid-state NMR using rotational resonance: implications for the supramolecular arrangement of the toxic conformer of Abeta42 |
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Authors: | Masuda Yuichi Nakanishi Azusa Ohashi Ryutaro Takegoshi Kiyonori Shimizu Takahiko Shirasawa Takuji Irie Kazuhiro |
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Affiliation: | Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Japan. |
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Abstract: | Formation of the intermolecular beta-sheet is a key event in the aggregation of 42-residue amyloid-beta (Abeta42). We have recently identified a physiological and toxic conformer, the turn positions of which are slightly different from each other, in the aggregates of E22K-Abeta42 (one of the mutants related to cerebral amyloid angiopathy). However, it remains unclear whether the intermolecular beta-sheet in the E22K-Abeta42 aggregates is parallel or antiparallel. We prepared an equal mixture of E22K-Abeta42 aggregates labeled at C(alpha) and those labeled at C=O with (13)C, whose intermolecular (13)C-(13)C distance was estimated by solid-state NMR using rotational resonance (R2). The intermolecular proximity of beta-strands at positions 21 and 30 was less than 6 A, supporting the existence of the intermolecular parallel beta-sheet in the E22K-Abeta42 aggregates as well as in wild-type Abeta42 aggregates. The results also suggest that each conformer would not accumulate alternately, but form a relatively large assembly. |
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