Isolation and characterization of a Delta5-desaturase from Oblongichytrium sp |
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| Authors: | Kumon Yasuyuki Kamisaka Yasushi Tomita Nao Kimura Kazuyoshi Uemura Hiroshi Yokochi Toshihiro Yokoyama Rinka Honda Daiske |
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| Institution: | Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan. |
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| Abstract: | We isolated a cDNA clone with homology to known desaturase genes from Oblongichytrium sp., recently classified as a new genus of thraustochytrids (Labyrinthulomycetes), and found that it encoded Delta5-desaturase by its heterologous expression in yeast. The enzyme had higher activity toward 20:4n-3 than 20:3n-6, indicating that this Delta5-desaturase can be used in the production of n-3 polyunsaturated fatty acids in transgenic organisms. |
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