A PROCEDURE FOR THE MEASUREMENT OF HEMICHOLINIUM-3 IN BRAIN TISSUE |
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| Authors: | P Slater P D Stonier |
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| Institution: | Department of Physiology, University of Manchester, Manchester 13, England |
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| Abstract: | Abstract— The natural fluorescence of hemicholinium-3 (HC-3) was used to provide a method for determining its concentration in brain tissue following intraventricular administration of the compound to rats and rabbits. When hemicholinium-3 was perfused through the cerebral ventricles of the rabbit, the highest concentration was found in the pons-medulla oblongata. The effect of a number of compounds on the uptake of hemicholinium-3 in the brain was studied in rats. Choline and tetramethylammonium significantly reduced the uptake of HC-3 from the cerebral ventricles but tetraethylammonium, physostigmine and atropine had no effect. The results are discussed in relation to the known effects of hemicholinium-3 in the central nervous system. |
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