|
|||||
|
|
Retinoid-binding proteins are phosphorylated in vitro by soluble Ca+2- and phosphatidylserine-dependent protein kinase from mouse brain |
|
| Authors: | F O Cope J M Staller R A Mahsem R K Boutwell |
| Institution: | McArdle Laboratory for Cancer Research, The University of Wisconsin Medical School, Madison, WI 53706 USA |
| Abstract: | A direct radioimmunoassay of atrial natriuretic factor (ANF) has been developed. The method uses a synthetic 26 amino-acid fragment (8-33 ANF) of the native peptide. Antibodies have been prepared in rabbits immunized with the peptide coupled to thyroglobulin. The radiolabelled tracer prepared by iodination according to the Chloramine-T method has been purified by HPLC followed by affinity chromatography on Sepharose-4B anti-ANF. Dextran-coated charcoal has been used for separation of free from antibody bound radioactivity. Higher ANF content has been found in the right rat atrium than in the left. These results have been confirmed by bioassay. |
| Keywords: | TPA 12-O-tetradecanoylphorbol-13-acetate PK-C cRBP cellular retinol-binding protein cRABP cellular retinoic acid-binding protein RBP's retinoid-binding proteins PAG polyacrylamide gel SDS-PAG sodium dodecylsulfate-polyacrylamide gel PS phosphatidylserine |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ | |