| 紫外线诱变选育碱性蛋白酶高产菌株 |
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| 引用本文： | 徐威,周丽娜.紫外线诱变选育碱性蛋白酶高产菌株[J].微生物学杂志,1997,17(1):18-21. |
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| 作者姓名： | 徐威 周丽娜 |
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| 作者单位： | 沈阳药科大学微生物教研室!110015 |
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| 摘 要： | 本实验以枯草杆菌A4－3为出发菌株，发酵产生碱性蛋白酶，其野生型菌株产酶为2412u／ml。采用孢子热处理方法处理出发菌株孢子，得到变异株As—4，产酶2732．8u／ml。对As—4菌株进行紫外线绣变处理1min，得变异菌株AX—46，产酶为3213．8u／ml，且产酶能力稳定。
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| 关 键 词： | 碱性蛋白酶 枯草杆菌 紫外诱变 |
SCREENING OF ALKALOPHILIC BACTERIA WITH HIGH ALKALINE PROTEINASE BY UV TREATMENT |
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| Xu Wei et al..SCREENING OF ALKALOPHILIC BACTERIA WITH HIGH ALKALINE PROTEINASE BY UV TREATMENT[J].Journal of Microbiology,1997,17(1):18-21. |
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| Authors: | Xu Wei |
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| Abstract: | Bacillus subtilis A4-3 was used as the starting strain in the experiment. Through fermentation, the production of alkaline proteinase was 2412 u/ml. when the spore of Bacillus subtilis A4-3 was treated by heat mutant As-4 was obtained, and the production of alkaline proteinase was 2732. 8 u/ml. When As-4 had mutated by UV radiation for one minute, mutant AX-46 was obtained, the production of alkaline proteinase was 3213. 8 u/ml with stable production capability. |
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| Keywords: | Alkaline proteinase Bacillus subtilis UV treatment |
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