Expression of an immunoglobulin kappa light-chain gene in lymphoid cells using a bovine papilloma-virus-1 (BPV-1) vector |
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| Institution: | 1. Departments of Immunology (Tel. (416)978-6382) Medical Sciences Building, University of Toronto, Toronto, Ontario Canada M5S 1A8;2. Departments of Medical Genetics (Tel. (416)978-7145), Medical Sciences Building, University of Toronto, Toronto, Ontario Canada M5S 1A8;1. Department of Research, Breeding and Production of Laboratory Animals, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran;2. Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada;3. Animal Science Research Institute of Iran (ASRI), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran |
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| Abstract: | Bovine papillomavirus-1 (BPV-1) replicates extrachromosomally in certain murine cell lines, suggesting that vectors based on the BPV-1 replicon might provide a means of obtaining more uniform gene expression among independent transformants. We have tested such a vector for the expression in hybridoma cells of the immunoglobulin κ light-chain gene, but found that the level of expression varies greatly among transformants. Our results also indicate that in these transformants the vector has probably been incorporated into chromosomal DNA. |
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