Cloning and sequencing of the gene for the DNA-binding 17 K protein of Escherichia coli |
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| Affiliation: | 1. Wellcome Trust-MRC Institute of Metabolic Science, University of Cambridge, Cambridge, UK;2. Department of Paediatrics, University of Cambridge, Cambridge, UK;3. Department of Diabetes and Endocrinology, Cambridge University Hospitals NHS Foundation Trust, Cambridge, UK;4. Manchester University NHS Foundation Trust and University of Manchester, Manchester, UK;5. Jaeb Center for Health Research, Tampa, FL, USA;6. Royal Hospital for Sick Children, Edinburgh, UK;7. Leeds Children''s Hospital, Leeds, UK;8. International Diabetes Center, Minneapolis, MN, USA;9. Barbara Davis Center for Diabetes, University of Colorado Anschutz Medical Campus, Aurora, CO, USA;1. Institute of Developmental Biology and Neurobiology (IDN), Johannes Gutenberg Universität, 55128 Mainz, Germany;2. Institute of Molecular Biology (IMB), Ackermannweg 4, 55128 Mainz, Germany;3. Instituto de Ciencias Biomedicas Abel Salazar, Universidade do Porto, 4050-313 Porto, Portugal |
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| Abstract: | The skp gene encoding the 17 K protein, a basic DNA-binding nucleoid-associated protein of Escherichia coli, was cloned as part of a 2.3-kb genomic fragment. The gene was sequenced and a polypeptide of 161 amino acids (aa) was deduced from the nucleotide sequence. The primary translation product was processed by cutting off the N-terminal 20 aa residues, yielding a mature polypeptide of 141 aa. The Mr of the mature polypeptide was 15674. An E. coli transformant containing the skp gene on the plasmid pGAH317 was shown to overproduce the gene product some 20-fold. |
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