Cloning,nucleotide sequence and characterization of genes encoding naphthalene dioxygenase of Pseudomonas putida strain NCIB9816 |
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| Institution: | 1. Key Laboratory of Desert and Desertification, Cold and Arid Regions Environmental and Engineering Research Institute (CAREERI), Chinese Academy of Sciences (CAS), Lanzhou, China;2. Key Laboratory of Extreme Environmental Microbial Resources and Engineering, Gansu Province, China;3. State Key Laboratory of Cryospheric Sciences, CAREERI, CAS, Lanzhou, China;4. School of Chemical and Biological Engineering, Lanzhou Jiaotong University, Lanzhou, China;5. College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, China;1. Department of Chemical Engineering, I-Shou University, Kaohsiung County, Taiwan;2. Department of Electrical Engineering, I-Shou University, Kaohsiung County, Taiwan;1. Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;1. Institute of Applied Genetics, Department of Molecular and Medical Genetics, University of North Texas Health Science Center, 3500 Camp Bowie Boulevard, Fort Worth, TX 76107, USA;2. Department of Forensic Medicine, Hjelt Institute, P.O. Box 40, 00014 University of Helsinki, Helsinki, Finland;3. Center of Excellence in Genomic Medicine Research (CEGMR), King Abdulaziz University, Jeddah, Saudi Arabia;1. G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, Russia;2. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia;3. Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences, Perm, Russia |
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| Abstract: | We have cloned the naphthalene dioxygenase(ND)-coding genes from Pseudomonas putida strain NCIB9816 based on their ability to convert indole to indigo. The region coding for this enzyme activity was sequenced and three successive open reading frames were found. The corresponding gene products were identified using the T7 polymerase/promoter system. All of them are necessary for the ND activity. A comparison of the ND-coding genes with the ones coding for benzene dioxygenase revealed significant homology which was more pronounced at the nucleotide level than at the amino acid level. |
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