Identification and linkage mapping of the phsA gene of Aspergillus nidulans, where mutation affects growth and pigmentation of colonies in a temperature- and pH-dependent way |
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| Authors: | Cuadros S C Martinez-Rossi N M Rossi A |
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| Affiliation: | 1. Russian Academy of Sciences Saint-Petersburg Scientific-Research Centre for Ecological Safety RAS (SRCES RAS), ul. Korpusnaya, 18, 197110 Saint-Petersburg, Russia;2. All-Russia Research Institute for Agricultural Microbiology, Sh. Podbelskogo 3, Pushkin-8, 196608 Saint-Petersburg, Russia;1. University of Vermont College of Medicine, 89 Beaumont Avenue, Burlington, VT 05405-0068, United States;2. Department of Dermatology, University of Vermont Medical Center, 111 Colchester Avenue - MS:250SM1, Burlington, VT 05401, United States;1. Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD 21231;2. Department of Pathology, Massachusetts General Hospital, Boston, MA 02114;3. The Knights Templar Eye Foundation Professor of Ophthalmology, The Wilmer Eye Institute, Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD 21231;4. Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University School of Medicine, Baltimore, MD 21231 |
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| Abstract: | We report the isolation and characterization of a mutant strain of the mold Aspergillus nidulans showing an altered response to environmental pH, including a reduction in its pH range for growth and the production of a melanin-like pigment at alkaline pH. We also show that the mutant strain is not detergent-sensitive and that its acid sensitivity is osmotically remediable with 0.5 M NaCl or 1.0 M sorbitol. Furthermore, the mutant phenotype is temperature-remediable with respect to pigmentation, extent of conidiation and growth diameter, with the restoration of a wild-type phenotype to the mutant strain being observed at 28 degrees C. On the other hand, the severity of the mutant phenotype is increased at 40 degrees C. Genetic analysis shows that this pH- and temperature-sensitive mutation, named phsA1, is located on the right arm of linkage group I of A. nidulans, between pabaA and yA, and that mutation phsA1 is recessive. |
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| Keywords: | Aspergillus nidulans pH sensitivity Melanin-like pigment |
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