The use of the 2-aminobenzoic acid tag for oligosaccharide gel electrophoresis |
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Authors: | Huang Z Prickett T Potts M Helm R F |
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Affiliation: | Fralin Biotechnology Center, Virginia Tech, Blacksburg 24061, USA. |
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Abstract: | Gel electrophoresis of fluorophore labeled saccharides provides a rapid and reliable method to screen enzymatic and/or chemical treatments of polysaccharides and glycoconjugates, as well as a sensitive and efficient microscale method to separate and purify oligosaccharides for further analysis. A simple and inexpensive method of derivatization and analysis using 2-aminobenzoic acid (anthranilic acid, AA) is described and applied to the extracellular polysaccharide released by the desiccation tolerant cyanobacterium Nostoc commune DRH-1. The results of these analyses suggest a possible protective functionality of two pendent groups, as well as a potential relationship between these groups and the desiccation tolerance of the organism. |
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