Involvement of equine chorionic gonadotropin (eCG) carbohydrate side chains in its bioactivity; lessons from recombinant hormone expressed in insect cells |
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Authors: | Legardinier Sébastien Cahoreau Claire Klett Danièle Combarnous Yves |
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Institution: | Unité de Physiologie de la Reproduction et des Comportements, Institut National de la Recherche Agronomique (INRA), Centre National de la Recherche Scientifique (CNRS) et Université Fran?ois Rabelais de Tours, 37 380 Nouzilly, France. |
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Abstract: | Natural eCG consists of as much as 45% carbohydrate side chains. The present paper deals with the analysis of the roles of the N- and O-linked saccharides of this hormone in the different steps of its activity and its possible replacement by recombinant eCG expressed in baculovirus-insect cell systems. |
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