Flip-Flop HSV-BAC: bacterial artificial chromosome based system for rapid generation of recombinant herpes simplex virus vectors using two independent site-specific recombinases |
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| Authors: | Toshihiko Kuroda Robert L Martuza Tomoki Todo Samuel D Rabkin |
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| Institution: | (1) Molecular Neurosurgery Laboratory, Department of Neurosurgery, Massachusetts General Hospital/Harvard Medical School, 185 Cambridge St., CPZN-3800, Boston, MA 02114, USA;(2) Department of Neurosurgery, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113–8655, Japan |
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| Abstract: | Background Oncolytic herpes simplex virus (HSV) vectors that specifically replicate in and kill tumor cells sparing normal cells are
a promising cancer therapy. Traditionally, recombinant HSV vectors have been generated through homologous recombination between
the HSV genome and a recombination plasmid, which usually requires laborious screening or selection and can take several months.
Recent advances in bacterial artificial chromosome (BAC) technology have enabled cloning of the whole HSV genome as a BAC
plasmid and subsequent manipulation in E. coli. Thus, we sought a method to generate recombinant oncolytic HSV vectors more easily and quickly using BAC technology. |
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