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Genetic and physical mapping of recF in Escherichia coli K-12 |
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| Authors: | Lloyd Walter Ream Linda Margossian Alvin J Clark Flemming G Hansen and Kaspar von Meyenburg |
| Institution: | (1) Department of Molecular Biology, University of California, 94720 Berkeley, California, USA;(2) Department of Microbiology, The Technical University of Denmark, DK-2800 Lyngby Copenhagen, Denmark |
| Abstract: | Summary Two factor transductional crosses place recF at approximately 82 min on the E. coli chromosome; recF is highly cotransducible with dnaA and gyrB (cou). Transductional analysis with a series of  tna specialized transducing phages carrying chromosomal DNA from the tnaA region place recF between dnaA and gyrB. This analysis also indicates that a gene lying in the same region and producing an easily detectable protein (estimated MW of 45 kD) is dnaN and not recF. |
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