Tunicamycin--an inhibitor of yeast glycoprotein synthesis |
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Authors: | S C Kuo J O Lampen |
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Institution: | Institute of Microbiology, Rutgers University, New Brunswick, New Jersey, USA |
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Abstract: | Tunicamycin, a glucosamine-containing antibiotic, halted synthesis of the external glycoproteins invertase, acid phosphatase and mannan by yeast protoplasts within 30 min; formation of two intracellular proteins, alpha-glucosidase and alkaline phosphatase, and of glucan continued at the control rate for at least 60–80 min. No accumulation of mannan-free acid phosphatase or invertase was evident in treated cells. Utilization of hexoses and incorporation of 14C-amino acids into protein were not affected. Incorporation of 3H-glucosamine into trichloroacetic acid-insoluble products was only partially reduced. In yeast tunicamycin acts primarily as an inhibitor of glycoprotein synthesis and not of general glucosamine metabolism. |
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