A NOVEL PROTECTING STRATEGY FOR INTERNUCLEOSIDIC PHOSPHATE AND PHOSPHOROTHIOATE GROUPS

The utility of 2–(N–isopropyl–N–anisoylamino)ethyl group for protection of internucleosidic phosphate linkages in oligonucleotide synthesis was studied. The group demonstrated high coupling yields, favorable deprotection kinetics and a high hydrolytic stability of phosphoramidite building blocks. The mechanism of deprotection was established using a model phosphate triester.