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A STUDY OF THE EFFECTS OF STATIC AND EXTREMELY LOW FREQUENCY MAGNETIC FIELDS ON LIPID PEROXIDATION PRODUCTS IN SUBCELLULAR FIBROBLAST FRACTIONS
Abstract:Cultured fibroblasts isolated from murine livers by tissue trypsinization were exposed to a static magnetic field (0.490 T) and to extremely low frequency (ELF) magnetic field (50 Hz, 0.020 T). The cultures were exposed to magnetic fields on four consecutive days for exposure times of 2, 4, 8, 16, 32, and 64 min. After such exposures and obtaining of fibroblast subcellular fractions, lipid peroxidation product—malondialdehyde (MDA) was measured. Increased peroxidation of fibroblasts' membrane structures exposed to an ELF magnetic field was observed in subcellular fractions—microsomal, mitochondrial, and nuclear. No changes in peroxidation of membrane structures were found in fibroblasts exposed to a static magnetic field.
Keywords:Fibroblast cultures  Static magnetic field  ELF magnetic field  Lipid peroxidation  Malondialdehyde (MDA)  Cellular fractions—microsomal  mitochondrial and nuclear
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