Determination of the Absolute P-Configuration of a Phthalidyl-Phosphonate Thymidine-Thymidine Dimer

Abstract

Phthalidyl modified oligonucleotide thymidine-thymidine dimer building blocks were synthesized via the H-phosphonate-method. The compounds which are diastereomeric at the phosphorus atom were separated by chromatography and the absolute configuration at the phosphorus atoms was determined using ROE-experiments using the corresponding methyl-phosphonates.