A New Concept for DNA-Arrays

Abstract

We will insert a cleavage site in an oligodeoxynucleotide, which can be used for a selective and quantitative cleavage. For that reason we synthesized the four 5′-S-(4,4′-dimethoxytrityl)-mercapto-2′-deoxynucleotide-3′-O-(2-cyanoethoxydiisopropylamino)-phosphites ( 5a–d ). The cleavage of P-S and C-S bonds is described (Mag, M.; Lücking, S.; Engels, J.W. Synthesis and selective cleavage of an oligodeoxy-nucleotide containing a bridged internucleotide 5′-phosphorthioate linkage Nucleic Acids Res. 1991, 19 (7), 1437–1441; Marriott, J.H.; Mottahedeh, M.; Reese, C.B. 9-(4-methoxyphenyl)xanthen-9-thiol: A useful reagent for the preperation of thiols. Tetrahedron Lett. 1990, 31 (51), 7485–7488; Divakar, K.J.; Mottoh, A.; Reese, C.B.; Shanghvi, Y.S. Approaches to the synthesis of 2′ thio analogues of pyrimidine ribosides. J. Chem. Sc., Perkin Trans. 1 1990, 969–974). The oligodeoxynucleotides with an achiral bridged 5′-phosphorothioate linkage 5′-O-P-S-3′ are synthesized by the phosphoramidite procedure.