PERYLENE ATTACHED TO DNA THROUGH STIFF OR FLEXIBLE LINKER: DUPLEX STABILITY AND FRET

Two fluorescent nucleosides, 5-(perylen-3-ylethynyl)-2′-deoxyuridine and 5-[(perylen-3-yl)methoxypropyn-1-yl]-2′-deoxyuridine, were incorporated into synthetic oligodeoxyribonucleotides and spectral properties of the conjugates and their duplexes were studied.