Denaturation time of actomyosin exposed to different chemicals]

The frog sceleton muscle actomyosin denaturation time dependence on the concentration of salts (NaCl and CaCl2) and organic chemicals (carbohydrates, narcotics and alcohols) was investigated. The following effects were detected: phase change in denaturation time associated with the rise of concentration of chemicals under study; actomyosin stabilization effect; coincidence of concentrations giving rise to protein stability with those increasing the survival time of isolated frog sceleton muscles in vitro (literature data); denaturation effect of alcohols used, both in high and very low concentrations.