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The possible proton translocating activity of the mitochondrial uncoupling protein of brown adipose tissue. Reconstitution studies in liposomes |
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| Authors: | F Bouillaud D Ricquier T Gulik-Krzywicki C M Gary-Bobo |
| Affiliation: | 1. Laboratoire de Physiologie Comparée (C.N.R.S. - L.A. 307), Université Pierre et Marie Curie, 4, Place Jussieu, F-75230 Paris Cedex 05, France;2. Centre de Génétique Moléculaire du C.N.R.S., F-91190 Gif-sur-Yvette, France;3. Départment de Biophysique, Centre d''Etudes Nucléaires de Saclay, F-91190 Gif-sur-Yvette, France |
| Abstract: | Loose coupling of thermogenic mitochondria of brown adipose tissue is related to a high proton (or hydroxyl) conductance of the inner membrane and to the presence of a unique 32 kDa uncoupling protein. Reconstitution experiments of the purified protein in liposomes are reported which suggest that this component could form proton channels in the membrane. |
| Keywords: | Uncoupling protein Reconstitution Proton translocation Phospholipid vesicle Freeze-fracture microscopy Brown adipose tissue Mitochondira |
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