海口市污水处理厂活性污泥中细菌的分离鉴定及耐药性分析

为了解海口市白沙门污水处理厂活性污泥中细菌抗生素耐性情况,采用平板分离技术分离、纯化细菌,并通过BIOLOG微生物鉴定系统对筛选到的细菌进行鉴定,同时采用Kirby-Bauer纸片琼脂扩散法进行药敏试验并进行抗生素耐性分析。本研究共分离到18株细菌,分属8个属,14个种,其中G+和G-均为9株。抗生素药敏性试验结果表明,所有菌株均耐药,菌株单重耐药率、双重耐药性及多重耐药性分别为50%、38.9%、和11.1%。菌株对9种常用抗生素:头孢他啶、环丙沙星、庆大霉素、链霉素、氨苄西林、红霉素、氯霉素、四环素、卡那霉素的耐药率分别为61.1%、0%、5.6%、16.7%、50%、16.7%、11.1%、0%、5.6%。综上所述,白沙门污水处理厂活性污泥中的细菌耐药性比较严重,存在潜在的环境生态和人畜健康风险。本研究揭示了当前白沙门污水处理厂活性污泥中细菌对常见抗生素耐药的严重现状,为建议污水处理厂加强出水及污泥中抗生素耐药性及耐药基因的检测并评估其生态影响提供基础,避免出水及污泥中的抗性菌和耐药基因可能带来的风险问题。

Isolation,Identification and Drug Resistance Analysis of Bacteria from Activated Sludge in Haikou Sewage Treatment Plant

In order to understand the bacterial antibiotic tolerance in the activated sludge of Baishamen sewage treatment plant in Haikou, the bacteria were isolated and purified by microbial plate separation technology, and the selected bacteria were identified by BIOLOG microbial identification system. Using the Kirby Bauer-paper agar diffusion method to improve the drug sensitive test and analysis of antibiotic resistance. A total of 18 strains of bacteria were isolated in this study, belonging to 8 genera and 14 species, of which 9 were G+and G-. Antibiotic susceptibility test results showed that all strains were resistant, and the single-drug resistance rate, double-drug resistance rate and multiple-drug resistance rate were 50%, 38.9% and 11.1%, respectively. The resistance rates of strains to 9 commonly used antibiotics such as ceftazidime, ciprofloxacin, gentamicin, streptomycin, ampicillin,erythromycin, chloramphenicol, tetracycline, kanamycin were 61.1%, 0%, 5.6%, 16.7%,, 50%, 16.7%, 11.1%, 0%,5.6%, respectively. In summary, the bacterial resistance in the activated sludge of Baishamen sewage treatment plant is relatively serious, and there are potential environmental ecology, human and animal health risks. This study reveals the current serious situation of bacterial resistance to common antibiotics in activated sludge of Baishamen Sewage Treatment Plant, and provides a basis for suggesting that sewage treatment plants strengthen the detection of antibiotic resistance and resistance genes in water and sludge and assess their ecological impact,avoid the risk of resistant bacteria and resistant genes in effluent and sludge.