CXCL8及其受体的表达在慢性乙肝患者临床诊断中的意义

为了揭示慢性乙肝患者外周血中性粒细胞上CXCL8及其受体CXCR1和CXCR2的表达情况。本研究选取2015年1月至2017年12月在我院确诊并治疗的慢性乙肝患者126例,其中男性78例,女性48例;HBeAg(+)患者51例,HBeAg(-)患者75例,中性粒细胞内的HBV DNA(+)患者48例,HBV DNA(-)患者78例。另外,选取同期我院经病理诊断为肝细胞正常的肝移植供肝者30例作为对照组。检测各组受试者外周血中性粒细胞上的CXCL8、CXCR1和CXCR2的m RNA和蛋白表达。病理G分级为G3~G4的患者的平均CXCL8、CXCR1和CXCR2 mRNA表达水平均显著高于G1~G2患者。病理S分期为S3~S4患者的CXCL8、CXCR1和CXCR2 mRNA表达水平显著高于S0~S2患者。严重程度为重度的患者的CXCL8、CXCR1和CXCR2 mRNA表达水平显著高于轻中度患者。HBeAg(+)患者的CXCL8和CXCR1 mRNA表达水平显著高于HBeAg(-)患者,且HBV DNA(+)患者的CXCL8和CXCR1 m RNA表达水平显著高于HBV DNA(-)患者。研究结果表明,病例组患者的CXCL8、CXCR1和CXCR2蛋白的阳性表达率分别为89.68%、83.33%和58.73%,而健康对照组的为10.00%、23.33%和43.33%。病例组的CXCL8和CXCR1阳性表达率显著高于对照组,而病例组CXCR2蛋白阳性表达率与对照组无显著差异。初步结论表明,CXCL8及其受体CXCR1和CXCR2与患者临床病理分期及疾病严重程度密切相关。监测CXCL8与CXCRl的表达水平对慢性乙肝的早期诊断及肝细胞损伤程度的判断具有重要意义。

Significance of CXCL8 and Its Receptor Expression in Clinical Diagnosis of Chronic Hepatitis B

To reveal the expression of CXCL8 and its receptors CXCR1 and CXCR2 in peripheral blood neutrophils in patients with chronic hepatitis B,a total of 126 patients with chronic hepatitis B diagnosed and treated in our hospital from January 2015 to December 2017 were enrolled,including 78 males and 48 females;51 patients with HBeAg(+) and 75 patients with HBeAg(-).There were 48 patients with HBV DNA(+) and 78 patients with HBV DNA(-) in neutrophils.In addition,30 patients with liver transplantation who were diagnosed as normal liver cells by pathology in our hospital were selected as the control group.The m RNA and protein expression of CXCL8,CXCR1 and CXCR2 in peripheral blood neutrophils of each group of subjects were examined.The mean CXCL8,CXCR1,and CXCR2 mRNA expression levels in G3~G4 patients were significantly higher than in G1~G2 patients(pathological G grade).The expression levels of CXCL8,CXCR1 and CXCR2 m RNA in S3~S4 patients were significantly higher than those in S0~S2 patients(pathological S stage).CXCL8,CXCR1,and CXCR2 mRNA expression levels in patients with severe severity were significantly higher than in mild to moderate patients.The expression levels of CXCL8 and CXCR1 m RNA in HBe Ag(+) patients were significantly higher than those in HBe Ag(-) patients,and the expression levels of CXCL8 and CXCR1 mRNA in HBV DNA(+) patients were significantly higher than those in HBV DNA(-) patients.The positive expression rates of CXCL8,CXCR1 and CXCR2 proteins in the case group were 89.68%,83.33% and 58.73%,respectively,while those in the healthy control group were 10.00%,23.33% and 43.33%.The positive expression rates of CXCL8 and CXCR1 in the case group were significantly higher than those in the control group,while the positive expression rate of CXCR2 protein in the case group was not significantly different from the control group.CXCL8 and its receptors CXCR1 and CXCR2 are closely related to the clinician’s clinical pathological stage and disease severity.Preliminary results suggested that monitoring the expression levels of CXCL8 and CXCR1 should be important for the early diagnosis of chronic hepatitis B and the determination of the degree of hepatocyte injury.