| 荷斯坦牛BoLA-DRA基因SNPs筛选及生物信息学分析 |
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| 引用本文： | 刘丽霞,乔自林,张丽,欧阳霞辉,虎红红,付保强.荷斯坦牛BoLA-DRA基因SNPs筛选及生物信息学分析[J].基因组学与应用生物学,2020,39(1):59-63. |
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| 作者姓名： | 刘丽霞 乔自林 张丽 欧阳霞辉 虎红红 付保强 |
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| 作者单位： | 西北民族大学生命科学与工程学院,兰州,730030;西北民族大学生物医学研究中心,兰州,730030 |
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| 基金项目： | 国家自然科学基金;中央高校基本科研业务费专项西北民族大学项目;西北民族大学双一流引导专项 |
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| 摘 要： | 为了探讨BoLA-DRA基因多态性,本研究利用DNA混合池扩增产物直接测序的方法对荷斯坦牛BoLA-DRA基因编码区SNPs进行筛选,并利用生物信息学软件预测该基因m RNA二级结构。结果表明:荷斯坦牛BoLA-DRA基因编码区共有4个SNPs (exon2-A82T,exon2-G116A,exon2-C197T,exon2-C233A)。生物信息学预测结果显示,exon2-A82T、exon2-G116A和exon2-C233A增大了m RNA二级结构的稳定性,而exon2-C197T降低了mRNA二级结构的稳定性。本研究结果可为荷斯坦牛抗病和抗逆性能研究积累更多的分子遗传学数据,并为经济性状相关基因筛选提供理论依据。
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| 关 键 词： | 荷斯坦牛 DNA混合池 BoLA-DRA SNPS 生物信息学 |
SNPs Screening and Bioinformatics Analysis of BoLA-DRA Gene in Holstein Cattle |
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| Liu Lixia,Qiao Ziling,Zhang Li,Ouyang Xiahui,Hu Honghong,Fu Baoqiang.SNPs Screening and Bioinformatics Analysis of BoLA-DRA Gene in Holstein Cattle[J].Genomics and Applied Biology,2020,39(1):59-63. |
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| Authors: | Liu Lixia Qiao Ziling Zhang Li Ouyang Xiahui Hu Honghong Fu Baoqiang |
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| Institution: | (College of Life Science and Engineering,Northwest Minzu University,Lanzhou,730030;Biomedical Research Center,Northwest Minzu Universi-ty,Lanzliou,730030) |
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| Abstract: | To discuss polymorphism of BoLA-DRA gene,the single nucleotide polymorphism site(SNPs) of coding region of BoLA-DRA gene in Holstein cattle were screened by DNA pooled amplification and directly sequencing,and further secondary structure of m RNA were predicted based on the bioinformatics software.The results showed that 4 SNPs(exon2-A82 T,exon2-G116 A,exon2-C197 T and exon2-C233 A) were detected in Holstein cattle.The results of bioinformatics showed exon2-A82 T,exon2-G116 A and exon2-C233 A increased the stability of secondary structure of m RNA,while exon2-C197 T reduced the stability of secondary structure of mRNA.The results could accumulate more molecular genetic data for research of disease resistance and resisting performance,and provide theoretical basis for selection of economic trait correlation genes. |
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| Keywords: | Holstein cattle BoLA-DRA DNA pooling SNPs Bioinformatics |
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