A study of the strength and stability of gas vesicles isolated from a blue-green alga |
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Authors: | Barbara Buckland A. E. Walsby |
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Affiliation: | (1) Department of Biology, University of Umeå, Sweden |
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Abstract: | Summary Acid phosphatase was studied by means of electron microscope cytochemistry in glutaraldehyde-fixed myxamoebae of Dictyostelium discoideum grown on dead bacteria. The enzyme activity was localized to the digestive vacuoles in vegetative as well as in aggregating cells. Biochemical experiments showed that the enzyme was not inactivated by fixation in 2% purified glutaraldehyde.Abbreviations used NPP p-nitrophenyl phosphate - NP p-nitrophenol - GP -glycerophosphate - glc-6-P glucose-6-phosphate - Pi orthophosphate |
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