XCAP—C类似蛋白存在于蒜根端细胞核和染色体中

以抗XCAP_C抗体为探针 ,用SDS_PAGE、免疫印迹、免疫荧光和免疫电镜技术 ,对蒜 (AlliumsativaL .)根端细胞核、核骨架、染色体和染色体骨架进行研究。SDS_PAGE和免疫印迹结果表明 :细胞核中的 16 5kD多肽是XCAP_C类似蛋白 ,在核骨架中未检测到XCAP_C类似蛋白。免疫荧光和免疫电镜结果表明 :蒜细胞核、染色体和染色体骨架中含有XCAP_C类似蛋白 ,该蛋白位于细胞核中的染色质区域 ,但核骨架不含有XCAP_C类似蛋白。

XCAP-C-like Protein Existing in Nuclei and Chromosomes of Allium sativa

In studying the XCAP_C_like protein in the root meristematic cells of Allium sativa L., the nuclei were isolated from the cells and the nuclear matrices prepared. A 165 kD polypeptide, which is equivalent to XCAP_C in molecular weight, was demonstrated in the nuclei by SDS_PAGE, and was then proved to be an XCAP_C_like protein by Western blot using an anti_XCAP_C antiserum, but neither the polypeptide nor the XCAP_C_like protein was detected in the nuclear matrix. The nuclei, chromosomes and chromosome scaffolds were observed to emanate strong, specific fluorescence after labeled with the anti_XCAP_C antiserum and an FITC_conjugated secondary antibody, indicating their containment of the XCAP_C_like protein. It was confirmed by viewing with immunoelectron microscopy that the gold particles representing the localization of the XCAP_C_like protein were found to be mainly distributed in the condensed chromatin regions of the nuclei and chromosomes.