Mass fragmentographic determination of lofepramine and its metabolites in human plasma and urine using deuterated internal standards |
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Authors: | Kyuichi Matsubayashi Hideo Hakusui Mitsuji Sano |
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Affiliation: | Research Institute of Daiichi Seiyaku Co., Ltd. 2810 Minamifunabori-cho, Edogawa-ku, Tokyo Japan |
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Abstract: | A sensitive and specific method for the determination of lofepramine and its metabolites, desipramine and 2-hydroxydesipramine, in human plasma and urine is described. Lofepramine, desipramine and 2-hydroxydesipramine were derivatized to ethyl p-chlorobenzoate, the bis(heptafluorobutyryl) derivative and the N,O-bis(trifluoroacetyl) derivative, respectively, and then analysed by gas chromatography—mass fragmentography. Corresponding deuterated compounds were used as internal standards. Determination was possible at levels as low as 2 ng/ml for lofepramine and desipramine and 20 ng/ml for 2-hydroxydesipramine. |
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