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Glycosylation of Mouse DPP4 Plays a Role in Inhibiting Middle East Respiratory Syndrome Coronavirus Infection |
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| Authors: | Kayla M Peck Adam S Cockrell Boyd L Yount Trevor Scobey Ralph S Baric Mark T Heise |
| Institution: | aDepartment of Biology, University of North Carolina—Chapel Hill, Chapel Hill, North Carolina, USA;bGenetics, University of North Carolina—Chapel Hill, Chapel Hill, North Carolina, USA;cEpidemiology, University of North Carolina—Chapel Hill, Chapel Hill, North Carolina, USA;dMicrobiology and Immunology, University of North Carolina—Chapel Hill, Chapel Hill, North Carolina, USA |
| Abstract: | Middle East respiratory syndrome coronavirus (MERS-CoV) utilizes dipeptidyl peptidase 4 (DPP4) as an entry receptor. Mouse DPP4 (mDPP4) does not support MERS-CoV entry; however, changes at positions 288 and 330 can confer permissivity. Position 330 changes the charge and glycosylation state of mDPP4. We show that glycosylation is a major factor impacting DPP4 receptor function. These results provide insight into DPP4 species-specific differences impacting MERS-CoV host range and may inform MERS-CoV mouse model development. |
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