Molecular characterization of structural genes coding for a membrane bound hydrogenase in Methylococcus capsulatus (Bath) |
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| Authors: | R Csáki T Hanczár L Bodrossy JC Murrell KL Kovács |
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| Institution: | Department of Biotechnology, University of Szeged, Hungary. |
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| Abstract: | The first gene cluster encoding for a membrane bound NiFe] hydrogenase from a methanotroph, Methylococcus capsulatus (Bath), was cloned and sequenced. The cluster consisted of the structural genes hupS and hupL and accessory genes hupE, hupC and hupD. A DeltahupSL deletion mutant of Mc. capsulatus was constructed by marker exchange mutagenesis. Membrane associated hydrogenase activity disappeared. The membrane associated hydrogenase appeared to have a hydrogen uptake function in vivo. |
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| Keywords: | Membrane bound hydrogenase hup gene cluster [NiFe] hydrogenase Methanotrophic bacterium Methylococcus capsulatus (Bath) |
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