Binding of Extracellular Matrix Molecules by Enterococci |
| |
Authors: | Igor tyriak sa Ljungh |
| |
Institution: | Department of Microbiology, Institute of Animal Physiology, Slovak Academy of Sciences, Soltésovej 4-6, 040 01 Kosice, Slovakia. styriak@saske.sk |
| |
Abstract: | The bacterial surfaces of enterococci are not uniform. This fact is confirmed by several studies and by our results when great
differences between individual strains with regard to their cell surface hydrophobicity, binding of eight ECM (extracellular
matrix) molecules immobilized on latex beads and four selected ECM molecules in microtiter plates were observed. The strains
expressing high binding of ECM molecules (e.g., HJ 18, HJ 23, HJ 24, HJ 26, HJ 28, HJ 36, etc.) were found among Enterococcus faecalis and E. faecium by PAA (particle agglutination assay). On the other hand, weak ECM binders (e.g., HJ 21, HJ 32, HJ 34, HJ 38, HJ 39, HJ 42,
HJ 43) were also found. A direct correlation was found between porcine mucin and fetuin binding ability of eight selected
strains tested in microtiter plates and by PAA. Moreover, the influence of tunicamycin treatment was different because significant
(P < 0.001) blocking effect of tunicamycin was observed with two selected strains (HJ 26 and HJ 36), whereas two strains (HJ 18 and HJ
22) were not significantly affected in their fetuin binding. The treatment of six enterococcal strains with proteolytic enzymes,
pronase P, and trypsin, and with sodium metaperiodate also significantly (P < 0.001) decreased their fetuin binding. This suggests that both protein and carbohydrate moieties are involved in the binding of
immobilized fetuin. However, the influence of these chemicals on the fetuin binding by individual strains was different.
Received: 24 May 2002 / Accepted: 2 August 2002 |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|