Drug metabolism components in liver microsomes from Hypostomus punctatus, a Brazilian benthic fish (Cascudo) |
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| Authors: | J C Bastos V L Bastos P Burth R Harab M G de Luna A Rossini M V Faria |
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| Institution: | Departamento de Bioqu?mica e de Biologia Celular, Universidade do Estado do Rio de Janeiro, Brazil. |
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| Abstract: | 1. The major components of hepatic drug biotransformation system were identified in a Brazilian freshwater benthic fish. 2. Cytochrome P-450 difference spectra were obtained adding 0.02 mM phenazine ethosulphate and 2 mM ascorbate to microsomal suspensions. Basal levels of P-450 were high (0.9 nmol/mg of microsomal protein) and were not induced by 3-MC. 3. Microsomal NADPH-cytochrome C reductase activity was determined in presence of 1.3 x 10(-4) M NADPH, 3.3 x 10(-5) M cytochrome C, 1.0 x 10(-4) M EDTA, 66 micrograms of microsomal protein per ml in a 0.3 M Tris-HCl buffer, pH 8.6. Basal levels of NADPH-cytochrome C were 152.7 nmoles/min/mg of microsomal protein. |
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