Characterization of a conserved apoptotic marker expressed in Caenorhabditis elegans phagocytic cells |
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Authors: | Eisenhut Robin J Knox Daniel Hermann Greg J |
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Affiliation: | Department of Biology, Lewis and Clark College, Portland, OR 97219, USA. |
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Abstract: | We have identified and characterized a monoclonal antibody, F2-P3E3, that recognizes a Caenorhabditis elegans apoptotic epitope expressed within phagocytic cells, which is conserved in four other nematode species. In C. elegans, F2-P3E3 staining requires both programmed cell death and phagocytosis. We show that the F2-P3E3 epitope is expressed within embryonic intestinal cells, which act as phagocytes but do not undergo programmed cell death. F2-P3E3 staining is present within LMP-1::GFP labeled organelles in the intestinal primordium and is coincident with persistent DNA that has been phagocytosed in nuc-1(-) embryos, suggesting that it labels phagosomes. While apoptotic events are typically isolated in C. elegans, F2-P3E3 staining is commonly found within adjacent cells. This observation suggests that F2-P3E3 might recognize an epitope expressed in multiple cells in response to signals from a single corpse. F2-P3E3 represents a new tool for studying cell death in C. elegans. |
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Keywords: | Caenorhabditis elegans F2-P3E3 Programmed cell death Apoptosis Phagocytosis |
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