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A monoclonal antibody against human colonic adenoma recognizes difucosylated Type-2-blood-group chains |
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| Authors: | A. Brown T. Feizi H. C. Gooi M. J. Embleton J. K. Picard R. W. Baldwin |
| Affiliation: | 1. Cancer Research Campaign Laboratories, University of Nottingham, University Park, NG7 2RD, Nottingham, UK 2. Applied Immunochemistry Research Group, Clinical Research Centre, Watford Road, HAI 3UJ, Harrow, Middlesex, UK |
| Abstract: | A monoclonal antibody C14/1/46/10 showing preferential binding to membranes of human colorectal carcinomas over normal colon mucosae was obtained by immunization of mice with extra-nuclear membranes of a human colonic adenoma. Binding and inhibition of binding assays using blood cells or glycoproteins with known blood-group activities indicated that the antibody recognizes a carbohydrate antigen co-existing with the blood-group-H determinant: Fucα1→2Gal. Inhibition assays with structurally defined oligosaccharides showed that the antigenic determinant involves difucosylated Type-2-blood-group chains with the structure: |
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