Oligomeric forms of potato spindle tuber viroid (PSTV) and of its complementary RNA are present in nuclei isolated from viroid-infected potato cells |
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Authors: | Ellen Spiesmacher Hans-Peter Mühlbach Martina Schnölzer Bernd Haas Heinz L Sänger |
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Institution: | 1. Abteilung Viroidforschung, Max-Planck-Institut fur Biochemie, D-8033, Planegg-Martinsried, Federal Republic of Germany
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Abstract: | Different oligomeric forms of PSTV are detected in nuclei isolated from PSTV-infected potato cells by means of molecular hybridization, using as probes synthetic oligodeoxyribonucleotides with sequence specificity for (+)PSTV and for (?)PSTV. In addition to several species of longer-than-unit-length (?)PSTV molecules, two oligomeric forms os (+)PSTV are detected, which correspond in size to RNA strands of approximately two and three times viroid unit-length. They must be considered as the precursors os the circular and linear (+)PSTV monomers accumulating in the cell nucleus. |
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