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Detection by denaturing gradient gel electrophoresis of a new polymorphism in the apolipoprotein B gene |
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| Authors: | Maria Navajas Anne-Marie Laurent Jean-François Moreel Ashraf Ragab Jean-Pierre Cambou Gérard Cuny François Cambien Gérard Roizès |
| Affiliation: | (1) CNRS UPR 8402 and INSERM U.249, Institut de Biologie, Boulevard Henri IV, F-34060 Montpellier Cedex, France;(2) INSERM U.258, INSERM SC 7, Hôpital Broussais, 96. Rue Didot, 14 Paris Cedex, France;(3) INSERM U, 101, CHU Purpan, Place Baylac, F-31059 Toulouse Cedex, France |
| Abstract: | Summary The apolipoprotein B gene is subject to mutations that may be important in coronary heart diseases. We have used polymerase chain reaction and denaturin gradient gel electrophoresis to characterize a single nucleotide substitution in the apolipoprotein B gene. This mutation affects amino acid 4311 of the protein and converts asparagine to serine. It was found in 24% of the 81 unrelated individuals analyzed. Moreover, another mutation was detected by sequencing in a single individual. |
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