| 轮状病毒VP7基因的克隆及其植物表达载体的构建 |
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| 引用本文： | 柴晓杰,靳非,王宇航,王晓庆.轮状病毒VP7基因的克隆及其植物表达载体的构建[J].生物技术通报,2009(1). |
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| 作者姓名： | 柴晓杰 靳非 王宇航 王晓庆 |
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| 作者单位： | 大连水产学院生命科学与技术学院,大连,116023 |
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| 摘 要： | 应用聚合酶链式反应技术(PCR)扩增了轮状病毒VP7基因,并将其克隆到pMD18-Tvectr载体上,对重组子进行PCR检测和限制性内切酶分析,并测定了DNA全序列.结果表明,克隆片段全长为981 bp.将轮状病毒VP7基因定向的克隆到植物表达载体pBI121启动子下游,构建了植物表达载体.
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| 关 键 词： | 轮状病毒 克隆 植物表达栽体 |
Cloning and Construction of Plant Expression Vector Containing Rotavirus VP7 Gene |
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| Chai Xiaojie,Jin Fei,Wang Yuhang,Wang Xiaoqing.Cloning and Construction of Plant Expression Vector Containing Rotavirus VP7 Gene[J].Biotechnology Bulletin,2009(1). |
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| Authors: | Chai Xiaojie Jin Fei Wang Yuhang Wang Xiaoqing |
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| Abstract: | Rotavirus VP7 gene was obtained by PCR techinque and cloned into pMD18-Tvector. The recombinant clone was detected by PCR technique and analyzed by the restriction enzyme. A full length of cDNA gene was sequenced. The results showed that the length of the clone sequence was 981bp. Rotavirus VP7 gene was cloned into promoter downstream of plant expression vector pBI121 in orientation. |
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| Keywords: | PCR |
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