Synaptic clusters of MHC class II molecules induced on DCs by adhesion molecule-mediated initial T-cell scanning |
| |
Authors: | de la Fuente Hortensia Mittelbrunn María Sánchez-Martín Lorena Vicente-Manzanares Miguel Lamana Amalia Pardi Ruggero Cabañas Carlos Sánchez-Madrid Francisco |
| |
Affiliation: | Servicio de Inmunología, Hospital de la Princesa, Universidad Autónoma de Madrid, 28006 Madrid, Spain. |
| |
Abstract: | Initial adhesive contacts between T lymphocytes and dendritic cells (DCs) facilitate recognition of peptide-MHC complexes by the TCR. In this report, we studied the dynamic behavior of adhesion and Ag receptors on DCs during initial contacts with T-cells. Adhesion molecules LFA-1- and ICAM-1,3-GFP as well as MHC class II-GFP molecules were very rapidly concentrated at the DC contact area. Binding of ICAM-3, and ICAM-1 to a lesser extent, to LFA-1 expressed by mature but not immature DC, induced MHC-II clustering into the immune synapse. Also, ICAM-3 binding to DC induced the activation of the Vav1-Rac1 axis, a regulatory pathway involved in actin cytoskeleton reorganization, which was essential for MHC-II clustering on DCs. Our results support a model in which ICAM-mediated MHC-II clustering on DC constitutes a priming mechanism to enhance antigen presentation to T-cells. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|