Validation of a high-throughput fermentation system based on online monitoring of biomass and fluorescence in continuously shaken microtiter plates |
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| Authors: | Frank Kensy Emerson Zang Christian Faulhammer Rung-Kai Tan Jochen Büchs |
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| Institution: | 1. AVT, Biochemical Engineering, Sammelbau Biologie, RWTH Aachen University, Worringerweg 1, 52074, Aachen, Germany
2. m2p-labs GmbH, Forckenbeckstra?e 6, 52074, Aachen, Germany
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| Abstract: | Background An advanced version of a recently reported high-throughput fermentation system with online measurement, called BioLector,
and its validation is presented. The technology combines high-throughput screening and high-information content by applying
online monitoring of scattered light and fluorescence intensities in continuously shaken microtiter plates. Various examples
in calibration of the optical measurements, clone and media screening and promoter characterization are given. |
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