Fluorine-19 magnetic resonance angiography of the mouse

Purpose

To implement and characterize a fluorine-19 (¹⁹F) magnetic resonance imaging (MRI) technique and to test the hypothesis that the ¹⁹F MRI signal in steady state after intravenous injection of a perfluoro-15-crown-5 ether (PCE) emulsion may be exploited for angiography in a pre-clinical in vivo animal study.

Materials and Methods

In vitro at 9.4T, the detection limit of the PCE emulsion at a scan time of 10 min/slice was determined, after which the T₁ and T₂ of PCE in venous blood were measured. Permission from the local animal use committee was obtained for all animal experiments. 12 µl/g of PCE emulsion was intravenously injected in 11 mice. Gradient echo ¹H and ¹⁹F images were obtained at identical anatomical levels. Signal-to-noise (SNR) and contrast-to-noise (CNR) ratios were determined for 33 vessels in both the ¹⁹F and ¹H images, which was followed by vessel tracking to determine the vessel conspicuity for both modalities.

Results

In vitro, the detection limit was ～400 µM, while the ¹⁹F T₁ and T₂ were 1350±40 and 25±2 ms. The ¹⁹F MR angiograms selectively visualized the vasculature (and the liver parenchyma over time) while precisely coregistering with the ¹H images. Due to the lower SNR of ¹⁹F compared to ¹H (17±8 vs. 83±49, p<0.001), the ¹⁹F CNR was also lower at 15±8 vs. 52±35 (p<0.001). Vessel tracking demonstrated a significantly higher vessel sharpness in the ¹⁹F images (66±11 vs. 56±12, p?=?0.002).

Conclusion

¹⁹F magnetic resonance angiography of intravenously administered perfluorocarbon emulsions is feasible for a selective and exclusive visualization of the vasculature in vivo.