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Levels of Thermostable Direct Hemolysin Produced by Vibrio parahaemolyticus O3:K6 and Other Serovars Grown Anaerobically with the Presence of a Bile Acid
Authors:Ro Osawa  Eiji Arakawa  Tadayuki Okitsu  Shiro Yamai  Haruo Watanabe
Institution:(1) Department of Bioscience, Graduate School of Science and Technology, Kobe University, Rokko-dai 1-1-I, Nada-ku, Kobe, 657-8501, Japan, JP;(2) Department of Bacteriology, National Institute of Infectious Diseases, Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan, JP;(3) Department of Bacteriology and Pathology, Kanagawa Prefectural Public Health Laboratory, Nakao 1-1, Asahi-ku, Yokohama, 241-0815, Japan, JP
Abstract:Twenty-three V. parahaemolyticus strains, including 12 pandemic O3:K6 strains, were examined for their growth and production of thermostable direct hemolysin (TDH) under an anaerobic culture condition with or without presence of a bile acid, taurocholic acid (TCA). Both bacterial growth and TDH production were markedly enhanced by TCA for a majority of the strains, but the scale of the TDH production was disproportionately greater than that of the corresponding growth for 14 strains. Such enhancement was, however, not specific to the pandemic strains. Received: 27 August 2001 / Accepted: 15 October 2001
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