Functional and phenotypic analyses of interleukin 2-activated tumor-infiltrating lymphocytes |
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Authors: | Hiroki Yamaue Hiroshi Tanimura Takuya Tsunoda Makoto Iwahashi Masaji Tani Mikiko Tamai Masaya Inoue |
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Affiliation: | (1) Department of Gastroenterological Surgery, Wakayama Medical College, 27-Shichibancho, 640 Wakayama, Japan |
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Abstract: | The tumor-infiltrating lymphocytes (TILs) were cultured with interleukin 2 (IL-2) to induce the activated killer cells possessing autologous tumor-killing activity, and analysed their cell surface phenotypes and assessed anti-tumor killing activity. Furthermore, the activated TILs were transferred into 7 patients adoptively resulting in complete remission in a patient with pancreatic cancer and partial remission in another patient with gastric cancer.The cytotoxic activities of activated TILs at 3 weeks-incubation was 72 ± 15, 42 ± 26, 27 ± 21 and 25 ± 15% against K562, Daudi, KATO-III and autologous tumor, respectively. The negative selection method, indicated that the killer cells recognizing autologous tumor cells consisted of CD4- or CD8-positive T lymphocytes and CD16- or CD56-positive natural killer cells. The activated TILs could not only lyse cultured tumor cell lines, but also autologous tumor cells. |
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Keywords: | adoptive immunotherapy autologous tumor-killing activity cytotoxic T lymphocytes interleukin 2 natural killer cells tumor-infiltrating lymphocytes |
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