Growth of Cephalotaxus harringtonia plant-cell cultures |
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| Authors: | Paul J. Westgate Alden H. Emery Paul M. Hasegawa Peter F. Heinstein |
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| Affiliation: | (1) School of Chemical Engineering, Purdue University, 47907 West Lafayette, IN, USA;(2) Departments of Horticulture, Purdue University, 47907 West Lafayette, IN, USA;(3) Departments of Medicinal Chemistry, Purdue University, 47907 West Lafayette, IN, USA;(4) Present address: Laboratory of Renewable Resources Engineering, Purdue University, 47907 West Lafayette, IN, USA |
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| Abstract: | Summary Cell cultures of Cephalotaxus harringtonia were examined to characterize growth kinetics. The requirement for an undefined medium supplement (coconut water) was eliminated by maintaining high cell concentrations in semicontinuous and batch growth. Sucrose fed to batch-cultured cells was completely hydrolyzed and a diauxic growth pattern was observed corresponding to first glucose and then fructose uptake. Examination of increases in cell concentrations on the basis of fresh and dry weight showed that a substantial lag period existed between the initiation of substrate uptake and increases in cell volume. Specific growth rates were highest during periods of glucose uptake, but cell yields were comparable for the two sugars. In contrast, studies with glucose or fructose as the sole carbon source indicated that cell yields were significantly lower with fructose but specific growth rates were comparable for the two sugars.Offprint requests to: P. J. Westgate |
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