The appearance of photosynthetic proteins in developing maize leaves |
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Authors: | Stephen P Mayfield William C Taylor |
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Institution: | (1) Biology Department, University of Saskatchewan, S7N 0W0 Saskatoon, Sask., Canada;(2) Plant Biotechnology Institute, National Research Council, S7N 0W9 Saskatoon, Sask., Canada |
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Abstract: | Soybean (Glycine max (L.) Merr.) protoplasts have been surface-labelled with cationized ferritin, and the fate of the label has been followed ultrastructurally. Endocytosis of the label occurs via the coated-membrane system. The pathway followed by the label, once it has been taken into the interior of the protoplast, appears to be similar to that found during receptor-mediated endocytosis in animal cells. Cationized ferritin is first seen in coated vesicles but rapidly appears in smooth vesicles. Labelled, partially coated vesicles are occasionally observed, indicating that the smooth vesicles may have arisen by the uncoating of coated vesicles. Structures which eventually become labelled with cationized ferritin include multivesicular bodies, dictyosomes, large smooth vesicles, and a system of partially coated reticula.Abbreviation CF
cationized ferritin |
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Keywords: | Glycine (pinocytosis) Multivesicular body Pinocytosis Pit (coated) Plasma membrane Protoplast (pinocytosis) Vesicle (coated) |
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