Preparation and properties of fluorescein-labelled hyaluronate. |
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Authors: | A N de Belder K O Wik |
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Affiliation: | Dept. of Chemical Research, Pharmacia AB, Uppsala and Institute of Medical and Physiological Chemistry, Biomedical Center, University of Uppsala, Uppsala Sweden |
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Abstract: | Hyaluronate has been labelled with fluorescein groups by two procedures. Products with degrees of substitution ((d.s.) between 0.05 and 0.001 were obtained. Physico-chemical analysis (viscometry, gel chromatography, and measurements of sedimentation and diffusion coefficients) of the parent compound and the products showed that the labelling procedures caused only a limited degradation of the polysaccharide. |
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