Uric acid synthesis in Rhodnius prolixus |
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| Authors: | F M Barrett W G Friend |
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| Affiliation: | 1. Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, Jilin 130102, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;3. INRS-ETE/Institut National de la Recherche Scientifique – Eau Terre Environnement, 490 rue de la Couronne, G1K 9A9 Quebec City, Quebec, Canada;4. School of Renewable Natural Resources, Louisiana State University Agricultural Center, 227 Highland Road, Baton Rouge, LA 70803, USA;1. Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Rua 235 s/n, Setor Universitário CEP 74605050 Goiânia, Goiás, Brazil;2. Escola de Veterinária e Zootecnia, Universidade Federal de Goiás, Campus II, Samambaia, Cx. Postal 131, CEP 74001-970 Goiânia, Goiás, Brazil;3. Instituto Federal Goiano, Campus Urutaí, Rodovia Geraldo Silva Nascimento Km 2,5, CEP 75790-000 Urutaí, Goiás, Brazil;4. Biological Chemistry and Crop Protection Department, Rothamsted Research, West Common, Harpenden, Hertfordshire AL5 2JQ, United Kingdom;1. Grupo de Neuroetología de Insectos Vectores, Laboratorio Fisiología de Insectos, Instituto Biodiversidad y Biología Experimental y Aplicada (IBBEA, UBA-CONICET), Departamento Biodiversidad y Biología Experimental (DBBE), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Intendente Güiraldes 2160, C1428EGA, Buenos Aires, Argentina;2. Centro de Investigaciones de Plagas e Insecticidas, Unidad de Investigación y Desarrollo para la Defensa, UNIDEF-CONICET, San Juan Bautista de La Salle 4397, B1603ALO, Villa Martelli, Buenos Aires, Argentina |
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| Abstract: | Glycine-1-14C, glycine-2-14C, and sodium formate-14C were used to investigate the origin of the carbon atoms of uric acid in Rhodnius prolixus. As in other uricotelic organisms, the carboxyl carbon of glycine is incorporated into position 4 of uric acid, the alpha carbon of glycine goes to position 5, and the carbon of sodium formate is incorporated into positions 2 and 8.The carboxyl carbon of glycine is converted to CO2 approximately twenty-four times more rapidly than is the alpha carbon, and much of this CO2 is incorporated into carbon 6 of uric acid. The alpha carbon of glycine is used in the synthesis of formate.The use of isotopically labelled precursors to determine the origin of all five carbon atoms of uric acid has not previously been reported for any species of insect. |
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