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比色法测定Fenton反应产生的羟自由基
引用本文:贾之慎,邬建敏,唐孟成. 比色法测定Fenton反应产生的羟自由基[J]. 生物化学与生物物理进展, 1996, 23(2): 184-186
作者姓名:贾之慎  邬建敏  唐孟成
作者单位:浙江农业大学无机及分析化学教研室;浙江农业大学无机及分析化学教研室;浙江农业大学无机及分析化学教研室
摘    要:Fenton反应产生的羟自由基能与水杨酸生成羟基化产物2,3-二羟基苯甲酸,用比色法测定其含量能间接测定羟自由基的生成量.通过对测定条件的研究,得到最佳的测定方案.可作为一种简便的筛选羟自由基清除剂的方法

关 键 词:羟自由基   Fenton反应   比色法   水杨酸
收稿时间:1995-06-30
修稿时间:1995-11-20

Colorimetric Determination of Hydroxyl Radicals from Fenton Reaction
Jia Zhishen,Wu Jianmin and Tang Mengcheng. Colorimetric Determination of Hydroxyl Radicals from Fenton Reaction[J]. Progress In Biochemistry and Biophysics, 1996, 23(2): 184-186
Authors:Jia Zhishen  Wu Jianmin  Tang Mengcheng
Affiliation:Teaching Group of Inorganic and Analytic Chemistry, ZhejiangAgricultural University;Teaching Group of Inorganic and Analytic Chemistry, ZhejiangAgricultural University;Teaching Group of Inorganic and Analytic Chemistry, ZhejiangAgricultural University
Abstract:Hydroxyl radicals from Fenton reaction can react with salicylate to form 2,3-dihydroxybenzoate.The quantitative analysis of hydroxyl radicals can be done through the colorimetric determination of 2,3 -dihydroxybenzoate.The determination condition has been studied and a best determination program has been obtained. The test indicated that hydroxyl radicals from this system could be scavenged by reutin and quercitin.This system can be used as a convenient method for the selection of hydroxyl radical scavenger.
Keywords:hydroxyl radical   Fenton reaction   colorimetry   salicylate
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