Enhanced detoxification of organophosphates using recombinant Escherichia coli with co-expression of organophosphorus hydrolase and bacterial hemoglobin |
| |
| Authors: | Dong Gyun Kang Jaoon YH Kim Hyung Joon Cha |
| |
| Institution: | (1) Department of Chemical Engineering & Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang, 790-784, Korea |
| |
| Abstract: | A significantly improved, recombinant Escherichia coli has been developed to degrade the toxic organophosphorus compound, Paraoxon, to non-toxic materials by co-expression of organophosphorus hydrolase (OPH) under trc promoter and Vitreoscilla hemoglobin (VHb) under O2dependent nar promoter. VHb-expressing whole cells had significant enhancement of OPH activity (48%, 18.7 vs. 27.8 unit l–1) and bioconversion efficiency V
max/K
m (44%, 0.14 vs. 0.2 min–1) compared to VHb-free system. |
| |
| Keywords: | bacterial hemoglobin organophosphate organophosphorus hydrolase recombinant Escherichia coli whole cell bioconversion |
| 本文献已被 SpringerLink 等数据库收录！ |
