Synthesis of low molecular weight alginic acid nanoparticles through persulfate treatment as effective drug delivery system to manage drug resistant bacteria |
| |
Authors: | Dipankar Ghosh Arindam Pramanik Narattam Sikdar and Panchanan Pramanik |
| |
Institution: | (1) Ecole Nationale Sup?rieure de Chimie de Rennes, Universit? Europ?enne de Bretagne, CNRS UMR 6226, Avenue du G?n?ral Leclerc, CS 50837, 35708 Rennes Cedex 7, France;(2) CEVA – Centre d’Etude et de Valorisation des Algues, presqu’?le de Pen Lan, 22610 Pleubian, France |
| |
Abstract: | The purpose of this study was to prepare low molecular weight alginic acid (LMWA) nanoparticles by cation-induced, controlled
gelification of depolymerized alginic acid for effective drug delivery to drug resistant bacteria. The depolymerization reaction
was performed using potassium persulfate oxidation at an optimized condition. The optimized conditions for depolymerization
were anticipated to be 37°C, pH 4, 2 days reaction time, and a 0.075 M concentration of potassium persulphate containing 0.001
M silver nitrate in the final reaction mixture. Gel permeation chromatography showed depolymerized alginic acid had an average
molecular weight of 20.95 ± 0.49 kDa. Depolymerized alginic acid was also characterized for its structural integrity by X-ray
diffraction, nuclear magnetic resonance, and Fourier transform spectroscopy. Depolymerized alginic acid was used to prepare
low molecular weight nanoparticles with a particle size of 54 ± 0.41 nm, and a zetapotential of −32.2 ± 3.91 mV. The nanoparicles
were then subjected to tetracycline loading. In vitro drug loading and drug release efficiencies after 100 h were determined to be 66.56 ± 1.88 and 61.8 ± 0.141%, respectively.
Finally, the minimal inhibitory concentration and a putative mode of action for the tetracycline nanoparticles were determined
using tetracycline resistant bacteria, Escherichia coli XL-1. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|